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CANCER IMMUNOLOGY GROUP MEMBERS

Dr. Ramziya Kiyamova
Ramziya Kiyamova
   

Education:

1980–1985

 

Master of Science in Genetics; Faculty of Biology, Kazan State University, Russia

1985–1990

 

Ph.D. student in Plant Physiology; Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre of Russian Academy of Sciences, Russia

   

Degree:

1992

 

Ph.D. in Plant Physiology; Thesis: “ Some structural and functional peculiarities of calluses of pea and soybean with different morphogenic potential”

   

Employment:

1990–1996

 

The Head of biotechnology laboratory in the National Agrarian University, Ukrainian Academy of Agriculture, Kyiv, Ukraine

1996-1998

 

Senior research scientist in “Diaprof LTD” biotechnological company, Kyiv, Ukraine

1998-1999

 

The Head of laboratory in “Oncotest LTD” biotechnological company, Kyiv, Ukraine

1999–present

 

Senior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation

2005

 

Visiting Scientist; Department of Biochemistry and Molecular Biology, University College London, London, UK

   

Skills in:

   

DNA cloning and protein purification in E.coli. Bac-to-Bac cloning, baculovirus generation and protein purification. Cell culture: transfection of mammalian and fly cell lines, maintenance of mouse, human and fly cell lines. Northern and Western blot analysis. Immunoprecipitation. ELISA. Oligo design and PCR. RT-PCR. Site-directed mutagenesis. SDS PAGE . RNA isolation, generation of cDNA libraries, cDNA library hybridization screening. Plant cell cultures and protoplast isolation techniques. Light and electronic microscopy: cytological, histological and cytogenetic analysis of plant cell cultures.

   

E.mail:
r.g.kiyamova@imbg.org.ua

kiyamova@mail.ru

 

Dr.Oleg Garifulin
   

Education:

1988–1993

 

Master of Science in Biochemistry National Taras Shevchenko University of Kyiv, Ukraine

1993–1996

 

Ph.D. student, Institute of Molecular Biology and Genetics of NAS of Ukraine

   

Degree:

2001

 

Ph.D. in Molecular Biology. Thesis: "Identification of gene complexes with altered normal expression in astrocytic gliomas".

   

Employment:

1996–present

 

Research scientist, Institute of Molecular Biology and Geneitcs of NAS of Ukraine

   

Skills in:

   

DNA cloning, DNA Sequencing, PCR, RT-PCR, construction of genomic and cDNA libraries, Southern and Northern dot and blot-analysis. RNA: isolation, cDNA synthesis, in vitro transcription/translation. cDNA: library construction and immunoscreening (SEREX methodology), hybridization screening, subtraction and normalization, differential display, "electronic" subtraction (digital differential display, SAGE-data analysis). Protein: SDS PAGE, Western Blots, Immunoprecipitation, bacterial expression, ELISA. Cell Culture: transient transfections, CAT assays, retroviral infections, growth of rat glioma cells, human glioblastoma cells and astrocytes, fibroblasts, glioma cell lines inoculation into rat brain.

   

E.mail: garifulin_o@yahoo.co.uk

 

Vitalina Gryshkova
   

Education:

1999–2003

 

Bachelor of science in Biology, Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine

2003–2005

 

Master of science in Immunology, Virology, Microbiology, Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine

   

Employment:

2002-2003

 

Associated student; Institute of Molecular Biology and Genetics, NAS of Ukraine, Laboratory of molecular mechanisms of autoimmune diseases

2003-2005

 

Associated student; Institute of Molecular Biology and Genetics, NAS of Ukraine, Laboratory of Cell Growth Regulation

2005–present

 

Junior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation

2007

 

Visiting Scientist; Department of Biochemistry and Molecular Biology, University College London, London, UK

   

Skills in:

   

DNA cloning , PCR, RT-PCR , SEREX-methodology, baculovirus expression system, bacterial and insect expression , purification of recombinant protein, SDS-PAGE, Western-blot analysis, immunoprecipitation, immunocytochemistry, ELISA , cell culture techniques, creation of stable cell lines, hybridoma technology.

   

E.mail: v.s.gryshkova@imbg.org.ua , vitalinochka@ukr.net


Sergei Malyuchik
   

Education:

2002-2007

 

Master of Science in Biochemistry National Taras Shevchenko University of Kyiv, Ukraine

   

Employment:

2004–2007

 

Associated student; Institute of Molecular Biology and Genetic of NAS of Ukraine, Laboratory of Cell Growth Regulation

2007–present

 

Lab technician; Institute of Molecular Biology and Genetic of NAS of Ukraine, Laboratory of Cell Growth Regulation

   

Skills in:

   

DNA cloning and protein purification in E.coli. Hybridoma technique. Western blot analysis. Immunoprecipitation. ELISA. PCR. SDS PAGE

   

E.mail: malyuchik@ukr.net



Other groups of our laboratory: