CANCER IMMUNOLOGY GROUP MEMBERS
Dr. Ramziya Kiyamova
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Education: |
19801985 |
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Master of Science in Genetics; Faculty of Biology, Kazan State University, Russia
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19851990 |
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Ph.D. student in Plant Physiology; Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre of Russian Academy of Sciences, Russia |
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Degree: |
1992 |
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Ph.D. in Plant Physiology; Thesis: “ Some structural and functional peculiarities of calluses of pea and soybean with different morphogenic potential”
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Employment: |
19901996 |
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The Head of biotechnology laboratory in the National Agrarian University, Ukrainian Academy of Agriculture, Kyiv, Ukraine
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Senior research scientist in “Diaprof LTD” biotechnological company, Kyiv, Ukraine |
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The Head of laboratory in “Oncotest LTD” biotechnological company, Kyiv, Ukraine |
1999present |
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Senior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation |
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Visiting Scientist; Department of Biochemistry and Molecular Biology, University College London, London, UK |
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Skills in: |
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DNA cloning and protein purification in E.coli. Bac-to-Bac cloning, baculovirus generation and protein purification. Cell culture: transfection of mammalian and fly cell lines, maintenance of mouse, human and fly cell lines. Northern and Western blot analysis. Immunoprecipitation. ELISA. Oligo design and PCR. RT-PCR. Site-directed mutagenesis. SDS PAGE . RNA isolation, generation of cDNA libraries, cDNA library hybridization screening. Plant cell cultures and protoplast isolation techniques. Light and electronic microscopy: cytological, histological and cytogenetic analysis of plant cell cultures. |
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E.mail:
r.g.kiyamova@imbg.org.ua
kiyamova@mail.ru
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Dr.Oleg Garifulin
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Education: |
19881993 |
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Master of Science in Biochemistry National Taras Shevchenko University of Kyiv, Ukraine |
19931996 |
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Ph.D. student, Institute of Molecular Biology and Genetics of NAS of Ukraine |
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Degree: |
2001 |
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Ph.D. in Molecular Biology. Thesis: "Identification of gene complexes with altered normal expression in astrocytic gliomas". |
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Employment: |
1996present |
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Research scientist, Institute of Molecular Biology and Geneitcs of NAS of Ukraine |
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Skills in: |
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DNA cloning, DNA Sequencing, PCR, RT-PCR, construction of genomic and cDNA libraries, Southern and Northern dot and blot-analysis. RNA: isolation, cDNA synthesis, in vitro transcription/translation. cDNA: library construction and immunoscreening (SEREX methodology), hybridization screening, subtraction and normalization, differential display, "electronic" subtraction (digital differential display, SAGE-data analysis). Protein: SDS PAGE, Western Blots, Immunoprecipitation, bacterial expression, ELISA. Cell Culture: transient transfections, CAT assays, retroviral infections, growth of rat glioma cells, human glioblastoma cells and astrocytes, fibroblasts, glioma cell lines inoculation into rat brain. |
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E.mail: garifulin_o@yahoo.co.uk |
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Vitalina Gryshkova
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Education: |
19992003 |
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Bachelor of science in Biology, Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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20032005 |
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Master of science in Immunology, Virology, Microbiology, Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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Employment: |
2002-2003 |
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Associated student; Institute of Molecular Biology and Genetics, NAS of Ukraine, Laboratory of molecular mechanisms of autoimmune diseases |
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Associated student; Institute of Molecular Biology and Genetics, NAS of Ukraine, Laboratory of Cell Growth Regulation
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2005present |
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Junior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2007 |
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Visiting Scientist; Department of Biochemistry and Molecular Biology, University College London, London, UK |
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Skills in: |
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DNA cloning , PCR, RT-PCR , SEREX-methodology, baculovirus expression system, bacterial and insect expression , purification of recombinant protein, SDS-PAGE, Western-blot analysis, immunoprecipitation, immunocytochemistry, ELISA , cell culture techniques, creation of stable cell lines, hybridoma technology.
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E.mail: v.s.gryshkova@imbg.org.ua , vitalinochka@ukr.net |
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Sergei Malyuchik
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Education: |
2002-2007 |
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Master of Science in Biochemistry National Taras Shevchenko University of Kyiv, Ukraine
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Employment: |
20042007 |
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Associated student; Institute of Molecular Biology and Genetic of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2007present |
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Lab technician; Institute of Molecular Biology and Genetic of NAS of Ukraine, Laboratory of Cell Growth Regulation
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Skills in: |
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DNA cloning and protein purification in E.coli. Hybridoma technique. Western blot analysis. Immunoprecipitation. ELISA. PCR. SDS PAGE |
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E.mail: malyuchik@ukr.net |
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Other groups of our laboratory:
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