TUMOR SUPPRESSOR INVESTIGATION GROUP MEMBERS
Dr. Sergiy Palchevskyy
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Education: |
1988-1993 |
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Master of Science in Molecular Biology; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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1993-1996 |
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Ph.D. student in Molecular Biology; Institute of Molecular Biology and Genetics of NAS of Ukraine, Department of Translation Mechanisms of Genetic Information
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Degree: |
1998 |
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Ph.D. in Molecular Biology; Thesis: " Investigation of ATPase activity of ribosomal subunits from rabbit liver " |
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Employment: |
1992–1993 |
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Lab technician; Institute of Molecular Biology and Genetics of NAS of Ukraine, Department of Translation Mechanisms of Genetic Information |
| 1993–1996 |
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Ph.D. student; Institute of Molecular Biology and Genetics of NAS of Ukraine, Department of Translation Mechanisms of Genetic Information |
1997-1998 |
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Research Associate, Institute of Molecular Biology and Genetics of NAS of Ukraine, Department of Translation Mechanisms of Genetic Information
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1998-2000 |
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Junior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2000-2006 |
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Research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation |
2004 |
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Visiting Scientist; Department of Biochemistry and Molecular Biology, University College London, London, UK |
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| 2006-present |
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Senior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation |
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Skills in: |
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DNA cloning techniques. PCR. DNA sequencing. Site-directed mutagenesis. Western, Northern and Southern blot analysis. Yeast two-hybrid system. Hy bridoma technique . SEREX techniques: creation and screening of expression libraries. D evelopment of cell-free reconstructed protein-synthesizing systems. FPLC. UV-VIS absorption spectroscopy. Preparation and characterization of lipid vesicles. Bac-to Bac baculovirus generation and protein purification. E xpression and purification of recombinant proteins from E.coli. Creation of stable cell lines. Immunoprecipitation. ELISA. I soelectric focusing. SDS-PAGE.
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E.mail: s.s.palchevskyy@imbg.org.ua |
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Dr.Olena Gorbenko
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Education: |
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Master of Science in Cell Biology and Genetic Engineering; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine |
2001-2004 |
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Ph.D. student in Molecular Biology; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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Degree: |
2006 |
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Ph.D. in Molecular Biology; Thesis: "Identification and characterization of PTEN binding proteins".
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Employment: |
1998-1999 |
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Lab assistant; Plant Growing Institute of NAS of Ukraine. |
1999-2000 |
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Lab technician; Institute of Cell Biology and Genetic Engineering of NAS of Ukraine. |
2001-2004 |
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Ph.D. student; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2004 |
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Visiting Ph.D. student; Ludwig Institute for Cancer Research, London, UK |
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Visiting Ph.D. student; Department of Biochemistry and Molecular Biology, University College London, London, UK |
2005-2006 |
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Junior research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2007 |
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Visiting PostDoc; Department of Biochemistry and Molecular Biology, University College London, London, UK |
2007–present |
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Research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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Skills in: |
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Yeast two-hybrid system. DNA cloning and protein purification in E.coli. Bac-to-Bac cloning, baculovirus generation and protein purification. Cell culture: transfection of mammalian and fly cell lines, retrovirus generation and infection, maintenance of mouse, human, hamster and fly cell lines, hybridoma technique. Southern and Western blot analysis. Immunoprecipitation. ELISA. Oligo design and PCR. Site-directed mutagenesis. SDS PAGE . Agrobacterium transformation and generation of transgenic plants. |
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E.mail: o.m.gorbenko@imbg.org.ua, termite@ukr.net |
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Oksana Malanchuk
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Education: |
1998-2002 |
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Bachelor of science in immunology, virology and microbiology; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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2002-2004 |
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Master of science in immunology, virology and microbiology; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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2004-present |
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Ph.D. student in Molecular Biology; National Taras Shevchenko University of Kyiv joined with Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2002 |
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Ph.D.in molecular biology. Thesis: "Study of elements of tyrosine tRNA recognition by tyrosyl-tRNA synthetase from higher eukaryotes using site-directed mutagenesis". |
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Degree: |
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Ph.D. thesis viva is expected on May 2008 |
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Employment: |
2000-2004 |
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Associated student; Institute of Molecular Biology and Genetics, NAS of Ukraine, Laboratory of Cell Growth Regulation
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2004–present |
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Ph.D. student; National Taras Shevchenko University of Kyiv, Faculty of Biology joined with Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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Skills in: |
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Yeast two-hybrid system; oligo design and PCR; DNA cloning techniques, expression and affinity purification of recombinant proteins; prokaryotic, yeast and mammalian cell culture techniques; transient transfection of mammalian cell lines; SDS-PAGE, ELISA, immunobloting, immunoprecipitation, immunofluorescence, immunocytochemistry; generation and characterization of polyclonal and monoclonal antibodies (hybridoma technique); kinase and phosphatase assays.
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E.mail: o.m.malanchuk@imbg.org.ua, malanchook@ukr.net
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Dr.Iryna Tykhonkova
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Education: |
1994-1999 |
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Master of Science in Bochemistry; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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Degree: |
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Ph.D. in Molecular Biology; Thesis: "Search and characterization of tumor-associated antigens from thyroid cancer and melanoma " |
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Employment: |
1999–2006 |
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Junior scientific researcher; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation
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2006-present |
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Research scientist; Institute of Molecular Biology and Genetics of NAS of Ukraine, Laboratory of Cell Growth Regulation |
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Skills in: |
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Characterization tumor associated antigen: protein purification, DNA cloning, PCR, construction of cDNA libraries, RNA isolation, cDNA synthesis, in vitro transcription/translation. cDNA library construction and immunoscreening (SEREX methodology), immunoblotting and ELISA assays, Western blotting, studies of autoantibody genesis in human heart diseases.
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E.mail: conference@imbg.org.ua |
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Dr.Mykola Rodnin  |
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Education: |
1977–1982 |
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Master of Science in Bochemistry; Faculty of Biology, National Taras Shevchenko University of Kyiv, Ukraine
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1983–1986 |
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Ph.D. student, Institute of Molecular Biology and Genetics of NAS of Ukraine |
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Degree: |
1990 |
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Ph.D. in Molecular Biology. Thesis: "Determination of the primary structure of the granulosis virus protein infecting Agrotis segetum". |
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Employment: |
1981–present |
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Researcher, Institute of Molecular Biology and Geneitcs of NAS of Ukraine |
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Skills in: |
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Protein purification, protein analysis, digestion of the proteins, determination of the peptide and proteins primary srtucture. Studies of baculoviruses proteins (polyhedrins and granulines), primary structure, DNA cloning, PCR, construction of cDNA libraries, Southern and Northern dot and blot-analysis. RNA: isolation, cDNA synthesis, in vitro transcription/translation. cDNA library construction and immunoscreening (SEREX methodology). Immunoblotting and ELISA assays, studies of autoantibodygenesis in human heart diseases. |
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E.mail: rodnin@imbg.org.ua |
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Other groups of our laboratory:
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